正版 刺参感染与免疫学 李成华 海洋出版社 9787521008647 书籍

章 刺参的生物学特征及产业发展现状 ······································ 11.1 分类地位 ·························································································· 11.2 形态特征 ·························································································· 11.3 生态习性 ·························································································· 21.3.1 分布 ······················································································· 21.3.2 栖息环境 ················································································· 31.3.3 食性与生长 ·············································································· 51.4 生物学特性 ······················································································· 61.4.1 夏眠 ····················································································· 61.4.2 排脏与再生 ············································································ 61.4.3 自溶 ······················································································· 61.5 繁殖习性 ························································································· 71.5.1 生殖习性 ················································································· 71.5.2 胚胎及幼体发育 ········································································ 81.6 刺参产业发展现状 ············································································101.6.1 我国刺参产业分布格局 ······························································101.6.2 我国刺参产业发展阶段 ······························································121.6.3 我国刺参增养殖模式 ·································································12主要参考文献 ·························································································13第2 章 刺参主要疾病及病原 ···························································192.1 腐皮综合征 ······················································································222.1.1 流行特征 ················································································222.1.2 病症 ······················································································222.1.3 化皮刺参体壁组织特征及炎症反应 ···············································23ii刺参感染与免疫学2.1.4 病原 ······················································································242.2 急性口围肿胀症 ················································································272.3 烂边症 ····························································································282.4 耳状幼体烂胃病 ················································································292.5 化板症 ····························································································302.6 排脏症 ····························································································312.7 霉菌病 ····························································································322.8 刺参原生动物疾病 ·············································································332.9 其他疾病 ·························································································34主要参考文献··························································································35第3 章 刺参病原检测技术 ·······························································373.1 免疫学检测技术 ················································································373.1.1 免疫荧光检测方法 ····································································373.1.2 免疫酶技术 ·············································································393.2 核酸检测技术 ···················································································393.2.1 直接核酸杂交技术 ····································································403.2.2 特定基因片段的PCR 检测技术 ···················································413.2.3 16S rRNA 检测技术 ··································································413.3 未来刺参病原检测的发展方向 ······························································423.3.1 免疫PCR················································································423.3.2 PCR-ELISA ·············································································433.3.3 生物芯片 ················································································433.3.4 基于噬菌体的病原检测新技术 ·····················································44主要参考文献··························································································46第4 章 刺参病原致病因子 ·······························································494.1 病原菌黏附 ······················································································494.2 灿烂弧菌的致病因子 ··········································································53iii目 录4.2.1 金属蛋白酶Vsm ······································································534.2.2 铁离子吸收 ·············································································544.2.3 溶血素 ···················································································564.2.4 分泌系统 ················································································594.2.5 单ADP - 核糖转移酶 ································································604.3 灿烂弧菌致病力的调控因子 ·································································604.3.1 σ 因子对致病力的调控 ·······························································604.3.2 群体感应对致病力的调控 ···························································61主要参考文献··························································································62第5 章 刺参细胞免疫 ······································································655.1 细胞免疫概念 ···················································································655.2 体腔细胞分类 ···················································································665.3 体腔细胞的免疫功能 ··········································································685.3.1 体腔细胞的趋化作用 ·································································685.3.2 细胞杀伤功能 ··········································································695.3.3 吞噬功能 ················································································705.4 细胞死亡与免疫 ··············································································725.4.1 细胞凋亡 ················································································725.4.2 细胞自噬 ················································································745.4.3 细胞焦亡 ················································································76主要参考文献··························································································77第6 章 刺参免疫因子的高通量筛选和挖掘 ·····································816.1 基因组 ····························································································816.2 表观组学 ·························································································826.3 转录组学 ·························································································846.3.1 mRNA ···················································································846.3.2 miRNA ···················································································93iv刺参感染与免疫学6.3.3 circRNA ·················································································956.3.4 lncRNA ················································································ 1006.4 蛋白组学 ······················································································· 1016.4.1 双向电泳技术 ········································································ 1016.4.2 同位素标记相对和保证定量技术 ················································ 1076.5 代谢组学 ······················································································· 111主要参考文献························································································ 115第7 章 刺参免疫因子功能 ····························································· 1197.1 Toll 样受体信号通路 ········································································ 1197.1.1 刺参Toll 样受体信号通路的组成 ················································ 1207.1.2 Toll 样受体的克隆及功能研究 ··················································· 1217.2 Toll 样受体信号通路的激活 ······························································· 1227.2.1 Toll 样受体信号通路关键接头分子鉴定 ······································· 1227.2.2 IRAK-1 和IRAK-4 的功能解析 ·················································· 1307.2.3 Toll 信号通路中的负调控因子 ··················································· 1347.2.4 NF-κB 亚基的鉴定及功能分析 ··················································· 1357.3 刺参IL17 信号通路 ········································································· 1377.3.1 IL17 信号通路组成 ································································· 1377.3.2 IL17 分子鉴定及功能研究 ························································ 1387.3.3 IL17 受体鉴定及功能研究 ························································ 1427.3.4 IL17 信号激活途径 ································································· 1437.4 JAK/STAT 信号通路 ········································································· 1457.4.1 JAK/STAT 信号通路的构成 ······················································· 1457.4.2 JAK/STAT 信号通路的调控 ······················································ 1467.4.3 刺参JAK/STAT 信号通路 ························································· 1467.5 Integrin 信号通路············································································· 1477.5.1 Integrin 的结构组成 ································································· 1477.5.2 Integrin 的信号转导机制 ··························································· 1487.5.3 刺参Integrin 信号通路 ····························································· 148主要参考文献························································································ 152v目 录第8 章 刺参非编码RNA 免疫功能研究 ······································· 1558.1 刺参miRNA 功能 ············································································ 1558.1.1 miRNA 概述 ·········································································· 1558.1.2 基于RNA-seq 的miR-92a 靶基因预测及其功能分析 ······················· 1568.1.3 基于RNA-seq 的miR-137/miR-2008 靶基因预测及其功能分析 ········· 1598.1.4 基于RNA-seq 的miR-31 靶基因预测及其功能分析 ························ 1688.1.5 miR-210 靶向TLR 介导刺参体腔细胞呼吸爆发 ····························· 1748.1.6 miR-210 靶向E2F3 基因调控刺参体腔细胞增殖 ···························· 1768.1.7 miR-133 靶向IRAK-1 基因调控刺参体腔细胞吞噬 ·························· 1778.2 刺参circRNA 功能 ·········································································· 182主要参考文献························································································ 189第9 章 刺参代谢免疫 ···································································· 1959.1 糖代谢对刺参免疫作用的影响 ···························································· 1959.1.1 刺参免疫应答过程中发生糖代谢重编程 ······································· 1959.1.2 刺参发生糖代谢重编程的调控机制 ············································· 1999.1.3 糖代谢重编程对刺参免疫应答的意义 ·········································· 2059.2 氨基酸代谢对刺参免疫作用的影响 ······················································ 2079.2.1 刺参免疫应答过程中发生精氨酸代谢 ·········································· 2079.2.2 精氨酸代谢与刺参免疫调控间的联系 ·········································· 2079.2.3 不同精氨酸代谢途径转录激活的差异 ·········································· 2129.2.4 灿烂弧菌精胺酶活性变化会影响刺参精氨酸代谢 ··························· 2169.2.5 精氨酸代谢对刺参免疫应答的意义 ············································· 218主要参考文献························································································ 219第10 章 刺参肠道微生物与疾病发生 ············································ 22310.1 刺参肠道微生物群落组成、结构和功能 ·············································· 22310.2 刺参肠道菌群的影响因素 ································································ 22710.2.1 地域 ················································································· 227vi刺参感染与免疫学10.2.2 季节和温度 ········································································ 22810.2.3 刺参的不同发育时期 ···························································· 22910.2.4 饲料添加剂 ········································································ 23110.3 刺参肠道微生物的功能 ································································ 23210.3.1 生物屏障作用 ····································································· 23210.3.2 提供营养，促进机体消化吸收 ················································ 23310.3.3 增强非特异性免疫 ······························································· 23310.3.4 促进肠道再生 ····································································· 23410.4 刺参肠道微生物与疾病 ··································································· 23510.5 环境因子协助肠道微生物促进疾病发生 ·············································· 238主要参考文献························································································ 244第11 章 刺参病害生态防治 ··························································· 24811.1 刺参抗病育种 ··············································································· 24811.2 免疫增强剂 ·················································································· 24911.2.1 中草药制剂 ········································································ 25011.2.2 免疫多糖类 ········································································ 25311.2.3 肽类物质 ··········································································· 25611.2.4 免疫增强菌剂 ····································································· 25611.3 拮抗菌剂 ····················································································· 26211.4 菌体和多糖类免疫增强剂的联合应用 ················································· 26611.5 “抗毒力”生物制剂的开发及应用 ······················································ 26611.6 噬菌体的开发与应用 ······································································ 268主要参考文献························································································ 272第12 章 总结展望 ·········································································· 279

李成华，博士，研究员，博士生导师，国务院学位委员会第八届水产学科评议组成员，国家很好青年科学基金获得者，“海洋牛物技术与工程”国家地方联合工程实验室主任，“水产生物技术”教育部重点实验室副主任，国家自然科学基金生命学部很好青年科学基金／学科评审组会评专家，国家外专局重点引智项目会评专家，Asian Society of Developmental and Comparative Immunology(ASDCI)理事，巾国海洋与湖沼学会棘皮动物分会理事，中同水产学会鱼病专业委员会委员。兼任靠前学术期刊Frontiers in Molecular Immunology主编，Fronttiers in Genetics、《水产学报》和《大连海洋大学学报》编委。入选2020年“爱思唯尔”中国高被引学者水产学科榜单，获浙江省“万人计划”科技创新领军人才、浙江省高校领军人才、曾呈奎海洋青年科技奖、中国水产学会首届青年科技奖、浙江省杰出青年科学基金、浙江省农业科技优选工作者、宁波市有突出贡献专家等荣誉和人才工程资助。主持国家重点研发计划课题和子课题各1项、同家自然科学基金5项以及浙江省自然科学基金杰出青年科学基金、重点基金和浙江省科技厅靠前合作项目等科研项目。以作者或通讯作者在PLoS Pathogens、Journal of Immunology、Genetics、Virulence、Review in Aquaculture、Environmental Microbiology等期刊发表SCI论文150余篇。以完成.人获2019年度高等学校科学研究很好成果奖(科学技术)自然科学奖二等奖(“刺参弧菌性疫病发生的分子基础及其生态防控机理”)、浙江省高等学校科研成果奖一等奖、宁波市科学技术奖三等奖和二等奖各1项。

《刺参感染与免疫学》为国内外第一部系统介绍刺参疾病病原和宿主免疫防御机制的专业书籍。该书在系统总结刺参生物学特征和产业发展现状的基础上，系统梳理了刺参主要疾病的流行病特征、病原类型和检测技术方法，进而聚焦危害最严重、死亡率最高和发病最广的刺参腐皮综合征发生的分子调控机制，结合李成华研究员团队在该领域十多年科研成果，同时衔接国内外刺参疾病与免疫学研究的最新进展，对宿主免疫防御机制与病原致病机制进行了系统描述。从免疫因子高通量发掘、免疫信号通路功能鉴定、代谢免疫和肠道微生物，体腔细胞的分类和免疫功能分化等方面阐述了宿主的体液免疫和细胞免疫防御机制。同时，聚焦灿烂弧菌等刺参腐皮综合征的典型病原的溶血素、金属蛋白酶、铁吸收等毒力因子阐释了病原的致病机制和调控途径。基于上述基础研究成果，从抗病育种、免疫增强剂和“抗毒力”生物制剂的开发以及噬菌体应用等方面深入探讨了刺参病害防治及生态防控策略。
 全书主旨明确，逻辑严谨，框架结构完整、配图精致清晰，可用作大专院校水产养殖专业辅助教材，也可作为水产动物免疫和病害防控等相关领域研究生的参考用书。