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  • abcam/艾博抗,ab83459,Aconitase Assay Kit,100tests
  • abcam/艾博抗,ab83459,Aconitase Assay Kit,100test
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    • 产品名称

      Aconitase Assay试剂盒

    • 检测方法

      Colorimetric
    • 样品类型

      Cell Lysate, Tissue Lysate
    • 检测类型

      Enzyme activity
    • 检测时间

      0h 40m
    • 产品概述

      Aconitase Assay Kit ab83459 is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring Aconitase activity in biological samples.


      In the aconitase assay protocol, citrate is converted by aconitase into isocitrate, which is further processed resulting in a product that converts a nearly colorless probe into an intensely colored form with a max absorbance at 450nm.


      Aconitase assay protocol summary:
      - activate aconitase in samples by adding cysteine HCl and (NH4)2Fe(SO4)2
      - add samples and standards to wells
      - add reaction mix and incubate for 30-60 min
      - analyze with a microplate reader

    • 说明

      This product is manufactured by BioVision, an Abcam company and was previously called K716 Aconitase Activity Colorimetric Assay Kit. K716-100 is the same size as the 100 test size of ab83459.

      Aconitase (aconitate hydratase; EC 4.2.1.3) is an iron-sulfur protein containing an [Fe4S4]2 cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle, a non-redox-active process.

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