The book focuses on the application of microfluidic technology in the research of antibiotic resistance.It includes the use of microfluidic chips to analyze bacterial resistance characteristics and to visualize the spread of antibiotic resistance genes (ARGs) at the single-cell level.Additionally,the authors tried to explore the impact of environmental pollutants,such as antibiotics,heavy metals,anull
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Chapter 1 Microfluidics for rapid antibiotic susceptibility testing 1
1.1 Microfluidic platform design 2
1.1.1 System design of microfluidic platform 2
1.1.2 Construction of microfluidic platform 3
1.1.3 Preparation of microfluidic agarose chip 4
1.1.4 Characterization of concentration gradient on the chip 8
1.2 Observation and data processing 9
1.2.1 Image processing and characterization for bacterial quantification 9
1.2.2 Microbial counting and growth model 10
1.2.3 Calculation of bacterial growth rate 11
1.2.4 Microbe inhibition dynamic model 11
1.3 Comparison of bacterial culture by chips and traditional methods 11
1.3.1 Growth of Escherichia coli in the chip and well plate 11
1.3.2 Growth of ammonia-oxidizing bacteria in the chips and shaking flasks 14
1.4 Amoxicillin susceptibility testing of different bacterial strains 16
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